›› 2012, Vol. 24 ›› Issue (2): 96-99.doi: 10.3969/j.issn.1004-616x.2012.02.004

• 论著 • Previous Articles     Next Articles

Detection of single nucleotide polymorphisms of DPYD and MTHFR genes in colon cancer cell lines

CHEN Bao-an，ZHANG Wen-jing，ZHANG Xiao-ping，BAI Zhi-bin，FENG Ji-feng，ZHONG Yue-jiao，DING Jia-hua ，GAO Chong， BAO Wen，PENG Miao-xin， CHENG Lu， LU Zu-hong   

  1. 1. Department of Hematology and Oncology, Affiliated Zhongda Hospital, School of Medical Science，Southeast University, Nanjing 210009; 2. Department of General Surgery, Affiliated Zhongda Hospital，School of Medical Science, Southeast University, Nanjing 210009; 3. Jiangsu Province Cancer Institute, Nanjing 210009, Jiangsu; 4. National Key Laboratory for Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, Jiangsu, China
  • Received:2011-06-09 Revised:2011-07-15 Online:2012-03-30 Published:2012-03-30
  • Contact: CHEN Bao-an

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Abstract: OBJECTIVE: This study was purposed to identify single nucleotide polymorphisms (SNPs) of two pharmacokinetics-related genes in two colon cancer cell lines. METHODS：HT-29 and Lovo cell lines were cultured. The genomic DNA of the two cell lines were isolated by QIAamp DNA Blood Mini kit. Then we designed the primers and amplified the related DNA fragments by PCR. SNP genotyping of DPYD gene rs1801159 (A/G)，rs1801160 (G/A)，rs17376848 (A/G) and MTHFR gene rs2274976 (G/A)，rs1801131 (A/C) and rs1801133 (C/T) was performed by means of matrix assisted laser desorption ionisation-time of flight mass spectrometry method (MALDI-TOFMS). RESULTS：The genotypes of DPYD gene locus rs1801159，rs1801160，rs17376848 were A/A，G/G and A/A，respectively，in the two cell lines. The genotype of MTHFR gene locus rs1801131，rs1801133 and rs2274976 were A/C，C/T and G/G，respectively，in HT-29 cell line. While those were A/A，T/T and G/A，respectively，in Lovo cell line. CONCLUSION： The above-mentioned loci of DPYD were the same in HT-29 and Lovo cell lines. However the detected loci of MTHFR gene in the two cell lines were expressed differently.

Key words: colon cancer, single nucleotide polymorphisms, DPYD, MTHFR